anti il 2rβ  (Bio X Cell)

Journal: Cell Reports Medicine

Article Title: Targeting severe acidity for tumor-activatable Interleukin-2 therapy

doi: 10.1016/j.xcrm.2025.102572

Figure Lengend Snippet: pH-responsive blocking and restoration of IL-2 receptor binding by UPS 5.3 (A) Schematic of the experimental design to assess the intrinsic interaction between UPS 5.3 micelles and proteins. (B) FPLC chromatograms showing individual proteins or UPS 5.3 /protein mixtures. (C) Molecular docking model of the DBA dimer (blue) with IL-2, highlighting predicted interaction sites. (D–F) Biolayer interferometry analysis of IL-2-Fc binding to IL-2Rα, IL-2Rβ, and IL-2Rγ, with and without UPS 5.3 NP encapsulation. (G) Schematic illustrating ex vivo assessment of IL-2-Fc binding to immune cells from various organs. (H) Quantification of IL-2-Fc binding to lymphocytes isolated from liver, lung, spleen, and tumor ( n = 5). Data are presented as mean ± SEM. See also and .

Article Snippet: InVivoMAb anti-mouse CD122 (IL-2Rβ) , BioXcell , Cat# BE0298 RRID: AB_2687820.

Techniques: Blocking Assay, Binding Assay, Encapsulation, Ex Vivo, Isolation

Journal: Advanced Science

Article Title: A Microbiota‐ and IL‐15‐Dependent Innate‐Like B Cell Progenitor Expressing E4BP4

doi: 10.1002/advs.202512444

Figure Lengend Snippet: NK‐B Cells Represent a Subset of B‐Cell Progenitors. A) Monocle trajectories of wild‐type bone marrow lymphocytes from Tabula Muris database colored by cluster identity. Each dot represents a single cell. Cell orders are inferred from the expression of the most variable genes across all cells. The trajectory direction was determined by biological prior. Green dots indicated stem cells, pink dots indicated NK‐B cells and blue dots indicated B cells. B) Monocle trajectories of wild‐type bone marrow CD3 − NK1.1 + cells colored by cluster identity. Each dot represents a single cell. Cell orders are inferred from the expression of the most variable genes across all cells. The trajectory direction was determined by biological prior. Green dots indicated NK‐B cells and blue dots indicated CD27 − CD11b − immature NK cells. C) Representative percentage and D) flow cytometric plots of NK1.1 expression in pro‐B cells (CD43 + IgM − ), pre‐B cells (CD43 − IgM − ), immature B cells (B220 int IgM + ) and mature B cells (B220 + IgM + ) in the bone marrow of the indicated mice (n = 3). E) Schematic representation. CD3 − NK1.1 + CD19 + NK‐B cells from CD45.1 + mice bone marrow were sorted and transferred into CD45.2 + Rag1 −/− γc −/− mice and analyzed at 2 months. F) NK cells (CD3 − CD19 − NK1.1 + CD122 + ), B cells (CD3 − CD19 + NK1.1 − ) and NK‐B cells (CD3 − CD19 + NK1.1 + ) existence was detected in recipient mice spleen and bone marrow. G) B cells development stage pro‐B cells (CD43 + IgM − ), pre‐B cells (CD43 − IgM − ), immature B cells (B220 int IgM + ) and mature B cells (B220 + IgM + ) existence was detected in recipient mice spleen and bone marrow (n = 3). H) Schemes depict four possible scenarios of NK‐B cell differentiation. Lymphocytes from indicated mice were isolated, stained with antibodies against CD3, CD19 and NK1.1 and analyzed by flow cytometry. CD3 − lymphocytes were gated out for analyzing NK1.1 versus CD19 I). Percentages of NK‐B cells (CD3 − CD19 + NK1.1 + ) J), NK cells (CD3 − CD19 − NK1.1 + ) K) and B cells (CD3 − CD19 + NK1.1 − ) L) were calculated (n = 3). Data represent the mean ± s.d. are representative of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Unpaired Student's t ‐tests (two‐tailed) was used to calculate these values.

Article Snippet: [ ] Mouse CD122 (TM‐beta 1, #BE0298) and IgG2b isotype control (LTF‐2, #BE0090) were purchased from BioXcell.

Techniques: Single Cell, Expressing, Cell Differentiation, Isolation, Staining, Flow Cytometry, Two Tailed Test

Journal: Advanced Science

Article Title: A Microbiota‐ and IL‐15‐Dependent Innate‐Like B Cell Progenitor Expressing E4BP4

doi: 10.1002/advs.202512444

Figure Lengend Snippet: IL‐15‐E4BP4 Axis and Microbiota Regulate NK‐B Cells Existence. A) Heat map illustrating the average transcript expression of the indicated genes (n = 3). The color key indicates the expression level. The expression levels of E4BP4, Eomes and T‐bet in B) NK cells (CD3 − CD19 − NK1.1 + ) and C) B cells (CD3 − CD19 + NK1.1 − ) from resting mice spleen and bone marrow. D) The expression levels of E4BP4, Eomes and T‐bet in NK‐B cells (CD3 − CD19 + NK1.1 + ) from resting mice spleen and bone marrow (n = 3). The expression levels of E4BP4 in NK‐B cells (CD3 − CD19 + NK1.1 + ) from E4BP4 reporter mice spleen and bone marrow stimulated with IL‐15 complex E,F) the absolute MFI (Zsgreen‐MFI) were quantified (n = 4). G) The expression levels of E4BP4 in NK cells (CD3 − CD19 − NK1.1 + ) and B cells (CD3 − CD19 + NK1.1 − ) from E4BP4 reporter mice spleen and bone marrow stimulated with IL‐15 complex. The absolute MFI (Zsgreen‐MFI) were quantified (n = 4). The expression levels of E4BP4 in CD122 + NK‐B cells and CD122 − NK‐B cells from E4BP4 reporter mice spleen and bone marrow stimulated with IL‐15 complex H,I) the absolute MFI (Zsgreen‐MFI) were quantified (n = 3). J) Absolute number of NK‐B (CD3 − CD19 + NK1.1 + ) (n = 5) and B (CD3 − CD19 + NK1.1 − ) (n = 4) cells in the spleen and bone marrow of the indicated mice. K) Absolute number of NK‐B (CD3 − CD19 + NK1.1 + ) cells in the spleen and bone marrow of the indicated mice (n = 5). Data represent the mean ± s.d. are representative of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Unpaired Student's t ‐tests (two‐tailed) was used to calculate these values.

Article Snippet: [ ] Mouse CD122 (TM‐beta 1, #BE0298) and IgG2b isotype control (LTF‐2, #BE0090) were purchased from BioXcell.

Techniques: Expressing, Two Tailed Test